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Expression of the alpha-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens
Cita
Carmona Quiles, Maria Jose, Molina Fernández, Antonio 
ORCID: https://orcid.org/0000-0003-3137-7938, Fernandez, Jose A., Lopez Fando, J.J. and García Olmedo, Francisco
(1993).
Expression of the alpha-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens.
"Plant Journal", v. 3
(n. 3);
pp. 457-462.
ISSN 0960-7412.
https://doi.org/10.1046/j.1365-313X.1993.t01-24-00999.x.
Descripción
| Título: | Expression of the alpha-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens |
|---|---|
| Autor/es: |
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| Tipo de Documento: | Artículo |
| Título de Revista/Publicación: | Plant Journal |
| Fecha: | Marzo 1993 |
| ISSN: | 0960-7412 |
| Volumen: | 3 |
| Materias: | |
| Escuela: | E.T.S.I. Agrónomos (UPM) [antigua denominación] |
| Departamento: | Biotecnologia [hasta 2014] |
| Licencias Creative Commons: | Reconocimiento - Sin obra derivada - No comercial |
Resumen
Thionins are cysteine-rich, 5 kDa polypeptides which are toxic to plant pathogens in vitro. Expression of the gene encoding alpha-thionin from barley endosperm, under the 35S promoter from cauliflower mosaic virus, conferred to transgenic tobacco enhanced resistance to the bacterial plant pathogens Pseudomonas syringae pv. tabaci 153 and P. syringae pv. syringae. The barley alpha-thionin gene, which has two introns, was correctly spliced in tobacco. The alpha-thionin in transgenic plants had the expected mobility in the gradient, when separated by high- performance liquid chromatography, reacted with monospecific antibodies and showed the expected antibiotic properties in vitro
Más información
| ID de Registro: | 6032 |
|---|---|
| Identificador DC: | https://oa.upm.es/6032/ |
| Identificador OAI: | oai:oa.upm.es:6032 |
| Identificador DOI: | 10.1046/j.1365-313X.1993.t01-24-00999.x |
| URL Oficial: | http://onlinelibrary.wiley.com/doi/10.1046/j.1365-... |
| Depositado por: | Memoria Investigacion |
| Depositado el: | 16 Feb 2011 12:03 |
| Ultima Modificación: | 20 Abr 2016 14:40 |
