Nat Methods

Abstract

The imminent release of tissue atlases combining multi-channel microscopy with single cell sequencing and other omics data from normal and diseased specimens creates an urgent need for data and metadata standards that guide data deposition, curation and release. We describe a Minimum Information about highly multiplexed Tissue Imaging (MITI) standard that applies best practices developed for genomics and other microscopy data to highly multiplexed tissue images and traditional histology.U54 CA225088/CA/NCI NIH HHSUnited States/U24 CA233243/CA/NCI NIH HHSUnited States/CSBC U54 CA225088/U.S. Department of Health & Human ServicesNIHNational Cancer Institute (NCI)/U2C CA233254/CA/NCI NIH HHSUnited States/U2C CA233238/CA/NCI NIH HHSUnited States/DRQ-03-20/Damon Runyon Cancer Research Foundation (Cancer Research Fund of the Damon Runyon-Walter Winchell Foundation)/U2C CA233280/CA/NCI NIH HHSUnited States/U2C CA233262/CA/NCI NIH HHSUnited States/01ZZ2004/Bundesministerium f\ufcr Bildung und Forschung (Federal Ministry of Education and Research)/U2C CA233284/CA/NCI NIH HHSUnited States/U2C CA233195/CA/NCI NIH HHSUnited States/U2C CA233311/CA/NCI NIH HHSUnited States/U2C CA233291/CA/NCI NIH HHSUnited States/1U24CA233243-01/U.S. Department of Health & Human ServicesOT2 OD026677/OD/NIH HHSUnited States/P2ZHP3_181475/Schweizerischer Nationalfonds zur F\uf6rderung der Wissenschaftlichen Forschung (Swiss National Science Foundation)/OT2 OD026677/CD/ODCDC CDC HHSUnited States/HHSN261201500003I/CA/NCI NIH HHSUnited States/U2C CA233285/CA/NCI NIH HHSUnited States/U2C CA233303/CA/NCI NIH HHSUnited States