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Motivation: A basic question in protein science is to which extent
mutations affect protein thermostability. This knowledge would be
particularly relevant for engineering thermostable enzymes. In several experimental approaches, this issue has been serendipitously addressed.It would be therefore convenient providing a computational method that predicts when a given protein mutant is more thermostable than its corresponding wild-type.
Results: We present a new method based on support vector machines that is able to predict if a set of mutations (including insertion and deletions) can enhance the thermostability of a given protein sequence. When trained and tested on a redundancy-reduced dataset, our predictor achieves 88% accuracy and a
correlation coefficient equal to 0.75. Our predictor also correctly
classifes 12 out of 14 experimentally characterized protein mutants
with enhanced thermostability. Finally, it correctly detects all the 11
mutated proteins whose increase in stability temperature is
Availability: The dataset and the list of protein clusters adopted for
the SVM cross-validation are available at the web sit
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