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PhoE, one of the outer membrane proteins
in E. coli K-12 cells, is involved in the
uptake of small hydrophilic, especially
negatively charged solutes. Its synthesis
is induced upon phosphate limitation. Several
models have been proposed for the mechanism
of transport and incorporation of
outer membrane proteins into the outer membrane. Escherichia coli cells containing
a hybrid phoE/lacZ gene were studied
to investigate the destination of the hybrid
gene product, using an immunocytochemical
labeling procedure and ultra-thin
cryosectioning
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