Pengaruh infusa daun murbei (Morus alba L.) terhadap gambaran histologi dan berat testis tikus putih (Rattus norvegicus) diabetes mellitus kronik

Abstract

INDONESIA: Daun murbei (Morus alba L.) merupakan salah satu tanaman tradisional yang mengandung senyawa antihiperglikemik dan antioksidan. Senyawa ini diketahui mampu menurunkan kadar glukosa darah yang tinggi dan memperbaiki kerusakan organ akibat peningkatan radikal bebas karena kondisi hiperglikemik. Penelitian ini bertujuan untuk mengetahui pengaruh infusa daun murbei (Morus alba L.) terhadap gambaran histologi testis dan berat testis tikus putih (Rattus norvegicus) Diabetes Mellitus Kronik. Penelitian ini bersifat eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 4 kali ulangan. Perlakuan yang digunakan adalah K (+) (kontrol positif), K (-) (kontrol negatif), P1 (400 mg/kg BB ), P2 (600 mg/kg BB), P3 (800 mg/kg BB) dan P4 (1000 mg/kg BB). Hewan yang digunakan adalah tikus putih jantan strain Wistar sebanyak 24 ekor yang berumur 2 bulan dengan berat rata-rata 70-100 gr. Data hasil penelitian meliputi jumlah sel germinal (spermatogonium, spermatosit primer, spermatosit skunder, spermatid dan spermatozoa), sel sertoli, diameter tubulus seminiferus, dan berat testis. Data yang diperoleh dianalisis dengan ANOVA One Way, apabila terdapat perbedaan sangat nyata maka dilanjutkan dengan uji lanjut Duncan α 1%. Hasil penelitian menunjukkan bahwa infusa daun murbei (Morus alba L.) berpengaruh dalam meningkatkan kembali jumlah sel germinal, sel sertoli, diameter tubulus seminiferus, dan berat testis tikus putih (Rattus norvegicus) diabetes mellitus kronik. Dosis optimal infusa daun murbei (Morus alba L.) yang berpengaruh dalam meningkatkan jumlah sel germinal, sel sertoli, diameter tubulus seminiferus, dan berat testis adalah P4 (1000 mg/kg BB). ENGLISH: Leaves of mulberry (Morusalba L.) is one of the traditional plants that contain compounds antihyperglycemicand antioxidant. These compounds are known to lower high blood glucose levels and improve organ damage due to free radicals due to an increase in hyperglycemic conditions. This study aims to determine the effect of infusion of mulberry leaves (Morusalba L.) on testicular weight and testicular histology white rat (Rattusnorvegicus) Chronic Diabetes Mellitus. This research was an experimental study using completely randomized design (CRD) with 6 treatments and 4 replications. The treatment used was K (+) (positive control), K (-) (negative control), P1 (400 mg / kg), P2 (600 mg / kg), P3 (800 mg / kg BW) and P4 (1000 mg / kg BW). Animals used were male Wistar strain rats tails were 24 ± 1 months old with an average weight of 70-100 grams. Research data include the number of germ cells (spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa), Sertoli cells, the diameter of the seminiferous tubules, and testes weight. Data were analyzed with One Way ANOVA, if there is a very real difference then followed by further test Duncan α 1%. The results showed that the infusion of leaves of mulberry (Morusalba L.) affectedon increasing the number of germinal cells, Sertoli cells, the diameter of the seminiferous tubules, and testis weight of white rat sufferingchronic diabetes mellitus. The optimum infusion dose of mulberry leaves (Morus alba L.) in increases the number of germ cells, Sertoli cells, the diameter of the seminiferous tubules, and testis weight was P4 optimal dose (1000 mg / kg BW)