Detection of Ply specific CD127^low/- FoxP3⁺ CD4⁺ Treg cells in CD25 enriched tonsil and blood MNC.

Abstract

<p>CD25 enriched MNC were stained with anti- CD127 and anti FoxP3 to allow identification of Tregs and with (<b>a</b>) strepavidin-PE, (<b>b</b>) negative control Ply tetramer-PE and (<b>c</b>) Ply tetramer-PE and analysed by FACS. A typical example of a FACS plot is shown. While the CD127^low/- FoxP3⁺ CD4⁺ Treg cells show low level staining at 0.0% with strepavidn-PE (<b>a</b>) and negative control Ply-tetramer at 0.05% (<b>b</b>), a significantly higher percentage of Treg cells are bound by Ply-tetramers at 1.96%(<b>c</b>).</p