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<p>(A–D) Thirty-six hours following transfection of COS-7 cells, hVAPB<sup>WT</sup> and hVAPB<sup>P56S</sup> mainly colocalize with components of the secretory pathway as demonstrated by the immunostaining of hVAPB with the ER marker KDEL (A), the COPI vesicle marker β-COP-CFP (B) and ERGIC marker ERGIC-53 (C). hVAPB<sup>P56S</sup> forms cytoplasmic aggregates that colocalize with ER, COPI and ERGIC markers. Both hVAPB<sup>WT</sup> and hVAPB<sup>P56S</sup> seldom colocalize (white arrow) with the COPII marker Sec23-YFP (D). Scale bar, 20 µm.</p
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