Additional file 5: of The M-phase specific hyperphosphorylation of Staufen2 involved the cyclin-dependent kinase CDK1

Abstract

STAU2 is phosphorylated by CDK1 during mitosis in HeLa cells. HeLa cells were incubated with nocodazole (Ndz) for 16 h in the absence (DMSO) or presence of specific kinase inhibitors for 4 h as indicated. Asynchronous (As) cells were used as controls. Cells were treated with the proteasome inhibitor MG132 (+) to keep cells in mitosis. STAU2 migration on SDS-gels was analyzed by western blotting. Cell markers were used to confirm specific inhibition of kinases. Western blots are representatives of three independently performed experiments that showed similar profiles. (PDF 422 kb