Repository landing page
Antifungal defense of probiotic <i>Lactobacillus rhamnosus</i> GG is mediated by blocking adhesion and nutrient depletion
Abstract
<div><p><i>Candida albicans</i> is an inhabitant of mucosal surfaces in healthy individuals but also the most common cause of fungal nosocomial blood stream infections, associated with high morbidity and mortality. As such life-threatening infections often disseminate from superficial mucosal infections we aimed to study the use of probiotic <i>Lactobacillus rhamnosus</i> GG (LGG) in prevention of mucosal <i>C</i>. <i>albicans</i> infections. Here, we demonstrate that LGG protects oral epithelial tissue from damage caused by <i>C</i>. <i>albicans</i> in our <i>in vitro</i> model of oral candidiasis. Furthermore, we provide insights into the mechanisms behind this protection and dissect direct and indirect effects of LGG on <i>C</i>. <i>albicans</i> pathogenicity. <i>C</i>. <i>albicans</i> viability was not affected by LGG. Instead, transcriptional profiling using RNA-Seq indicated dramatic metabolic reprogramming of <i>C</i>. <i>albicans</i>. Additionally, LGG had a significant impact on major virulence attributes, including adhesion, invasion, and hyphal extension, whose reduction, consequently, prevented epithelial damage. This was accompanied by glucose depletion and repression of ergosterol synthesis, caused by LGG, but also due to blocked adhesion sites. Therefore, LGG protects oral epithelia against <i>C</i>. <i>albicans</i> infection by preventing fungal adhesion, invasion and damage, driven, at least in parts, by metabolic reprogramming due to nutrient limitation caused by LGG.</p></div- Dataset
- Dataset
- Biochemistry
- Microbiology
- Molecular Biology
- Ecology
- Cancer
- Biological Sciences not elsewhere classified
- albicans viability
- adhesion sites
- ergosterol synthesis
- depletion Candida albicans
- albicans infection
- albicans infections
- virulence attributes
- epithelial tissue
- glucose depletion
- hyphal extension
- nosocomial blood stream infections
- mucosal surfaces
- mucosal infections
- albicans pathogenicity
- Antifungal defense
- LGG
- epithelial damage
- mucosal C
- probiotic Lactobacillus rhamnosus GG