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Lymphocyte changes in LN after hetIL-15 treatment.
Abstract
<p>(A) Step-dose regimen of six SC hetIL-15 administrations in rhesus macaques. LN, blood and mucosal tissue lymphocytes were analyzed before (pre) and after treatment (+hetIL-15). Flow cytometry dot plots of LN mononuclear cells show (B) the frequency of CD8<sup>+</sup> memory subsets, naïve (T<sub>N</sub>, CD28<sup>+</sup>CD95<sup>low</sup>), central memory (T<sub>CM</sub>, CD28<sup>high</sup>CD95<sup>+</sup>) and effector memory (T<sub>EM</sub>, CD28<sup>-</sup>CD95<sup>+</sup>), and (D) granzyme B content and cycling status (GrzB<sup>+</sup>Ki67<sup>+</sup>) from a representative uninfected macaque (R921) upon hetIL-15 treatment. Graphs (C, E, F) summarize results of 16 macaques treated with hetIL-15 of (C) frequency of effector memory CD8<sup>+</sup> T cells, (E) CD8<sup>+</sup>GrzB<sup>+</sup> T cells, and (F) cycling (Ki67+) CD8<sup>+</sup> T cells. Analysis was performed on LN of 9 uninfected animals (filled symbols) and 7 SHIV<sup>+</sup> macaques (open symbols). Black symbols, pre; red symbols, +hetIL-15. P values are from paired Wilcoxon signed rank test. The 12 animals that were also analyzed for hetIL-15 effects in blood and mucosal tissues (Figs <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006902#ppat.1006902.g002" target="_blank">2</a> and <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006902#ppat.1006902.g003" target="_blank">3</a>) are indicated by *.</p- Image
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- Biochemistry
- Medicine
- Cell Biology
- Molecular Biology
- Physiology
- Immunology
- Cancer
- Infectious Diseases
- Virology
- Chemical Sciences not elsewhere classified
- lymph nodes B cell follicles
- heterodimeric IL -15 increases cytotoxic lymphocytes
- SHIV RNA
- hetIL -15 treatment
- effector CD 8
- cytotoxic CD 8
- FH
- B cell follicles
- hetIL -15
- virus-specific MHC-peptide tetramer
- combination immunotherapy regimens
- LN
- heterodimeric IL -15
- T cells
- NCT
- target AIDS virus sanctuaries
- granzyme B content