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Poster PresentationIntroduction: Sry-related HMG box (SOX) genes
belong to a family of transcription factors sharing a
conserved high-mobility-group domain. However,
information about the functions of Group F sox
genes (sox 7, 17, 18 and sox32), particularly in
hematopoiesis, are scarce. This is addressed in
the present study using morpholino gene
knockdown in zebrafish.
Method: Morpholinos (MO) targeting selected
sox genes were injected into zebrafish embryos
and were analyzed for effects in hematopoiesis
using real time RT-PCR and WISH.
Results: Sox17 was expressed in neural tube
and intermediate cell mass (ICM) while sox7 and
sox18 was enriched in the vasculature. Sox32,
unique in teleosti, was expressed predominantly in
the posterior ICM. Of the four, only sox17 was
highly enriched in FAC sorted gata-1 positive cells.
Injection of Sox17 MO resulted in significant
decrease in alpha embryonic hemoglobin (Į-eHB)
and gata-1 expression which can be reconstituted
by co-injecting only sox17 transcripts, but not by
sox7, sox18 or sox32 transcripts. Co-injection of
Sox7 and Sox18 MO had no effect on Į-eHB
expression. Knockdown of sox32 resulted in
decrease of Į-eHB expression, likely to be due to
abnormal embryo patterning. Cell cycle profile of
gata-1 positive cells in sox17 morphants showed
an increase in the percentage of cells in the G1
phase. While either knockdown or over-expression
of this gene have no effect on ȕ-catenin signaling
as shown by the Tg(Top:GFP)w25 transgenic
zebrafish embryos.
Conclusion: Of the four SoxF genes in zebrafish,
sox17 have unique role in primitive erythropoiesis,
enhancing the transition of these cells through the
G1 phase. Unlike other orthologs, zebrafish sox17 have no effect on ȕ-catenin signaling. Therefore
this gene was unlikely to effect the proliferation of
erythroid cells in zebrafish embryos through this
pathway
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