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Extracellular vesicles (EVs) are particles naturally released from cells, delimited by a lipid
bilayer, carrying functionally active biological molecules. In addition to their physiological role in
cellular communication, the interest of the scientific community has recently turned to the use of
EVs as vehicles for delivering therapeutic molecules. Several attempts are being made to ameliorate
drug encapsulation and targeting, but these efforts are thwarted if the starting material does not
meet stringent quality criteria. Here, we take a step back to the sources and isolation procedures
that could guarantee significant improvements in the purification of EVs to be used as drug carriers,
highlighting the advantages and shortcomings of each approac
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