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This paper describe different techniques we can use for multiple sequence alignment. we have analyze different techniques we can use and find which one is better .We have taken several example as parameter. There is different approachthat we can apply in various conditions. This paper is concerned with the efficient execution Multiple sequence alignment methods in multiple client environments Multiple order alignment (MSA) is in a computational form Expensive method, which is commonly used in Large databases of computational and molecular biology Protein and gene sequences are available for scientists Community frequent, these databases are accessed to execute multiple user MSA queries Data is in server
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