Germ Line Chimera Production: Inspection Donor Primordial Germ Cells Transferred to Recipient Embryos

Abstract

Produce germ line chimeras in chickens has been possible by transferring donor Primordial Germ Cells (PGCs) to recipient embryos. The success of germ line chimera formation is one of the factors determining the presence and development of donor PGCs in recipient embryos. The aim of this study was to see be of donor PGCs in recipient embryos through staining and examination of embryo development. One hundred and fifty fertile eggs (100 KUB eggs and 50 WL eggs) incubated is in a portable incubator at 38oC temperature and 60% humidity. PGC-circulating collection of KUB chickens from 2.5-day old embryos (stages 15-16) purified by the ACK lysis buffer method. Staining performed is by visualizing donor cells using the PKH-26 Red Fluorescence Cell Linker Mini Kit. The PGCs are then transferred to the recipient embryo. The presence of PGCs and develop recipient embryos analyzed is descriptive. The results showed that through staining and examination, the presence of circulating PGCs into the recipient gonads was detected. It concluded is that germ line chimeras obtained, which indicated by the presence of PGCs in the recipient embryo