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oaioai:rune.une.edu.au:1959.11/19091

The Vector Construction of Anthocyanins as a Visual Marker Gene and Its Transient Expression in Maize Immature Embryos

Authors
Publication date
1 June 2016
Publisher
Zhongguo Nongye Kexueyuan

Abstract

采用花青素调控因子C1/Bperu分别与玉米胚特异性启动子Glb1和组成型启动子CaMV35S构建成植物转化载体pGlb1CB和p35SCB，并利用基因枪转化方法，将重组表达载体转入玉米幼胚。显微观察结果证实，这两个载体均能在玉米幼胚细胞中瞬时表达。用花青素作为标记基因不仅可以在一定程度上减少公众对转基因生物安全性方面的担忧，而且可以帮助直观地从转化当代和后代种子中通过颜色标记筛选到转化籽粒，从而可以大大简化筛选程序，提高效率，节约检测成本

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Last time updated on 09/08/2023

This paper was published in Research UNE.

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